QCM biosensor for Prostate Specific Antigen assay using antibody - gold particle conjugate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557067" target="_blank" >RIV/60162694:G44__/21:00557067 - isvavai.cz</a>

Result on the web

<a href="http://www.electrochemsci.org/abstracts/vol16/21051.pdf" target="_blank" >http://www.electrochemsci.org/abstracts/vol16/21051.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2021.05.04" target="_blank" >10.20964/2021.05.04</a>

Result language

angličtina

Original language name

QCM biosensor for Prostate Specific Antigen assay using antibody - gold particle conjugate

Original language description

Prostate Specific Antigen (PSA) is a key marker for prostate cancer screening by standard laboratory tests based on blood, plasma or serum analysis. The current laboratory methods for PSA level determination are based on various chromatographic, immunochemical and other instrumental analyses that has to be done in the specialized laboratories. Experiments in this work are focused on construction of a Quartz Crystal Microbalance (QCM) biosensor as a simple device suitable for point-of-care diagnosis based on PSA concentration determination in body fluids. The QCM sensors were covered with antibody against PSA and gold nanoparticles modified with specific antibody against PSA were prepared in a separate step. Change in oscillation frequency was measured as the immunocomplexes QCM biosensor - PSA - modified gold nanoparticles were formed. Calibrating for standard solution of PSA, limit of detection equal to 0.054 mu g/l and limit of quantification equal to 0.18 mu g/l were figured. The biosensor was not sensitive to interference of other plasma proteins or to matrix effect and fully correlated with the Enzyme-Linked Immunosorbent Assay (ELISA) as a standard method. In a conclusion, the biosensor appears to be a simple and reliable tool that can be used in standard laboratory and point-of-care testing.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

9

Pages from-to

21051

UT code for WoS article

000661483700001

EID of the result in the Scopus database

2-s2.0-85104748474