Heat Shock Protein 60 (HSP60) detection by QCM Biosensor and Antibody Covered Gold Nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557068" target="_blank" >RIV/60162694:G44__/21:00557068 - isvavai.cz</a>
Result on the web
<a href="http://www.electrochemsci.org/papers/vol16/210512.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol16/210512.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.20964/2021.05.11" target="_blank" >10.20964/2021.05.11</a>
Alternative languages
Result language
angličtina
Original language name
Heat Shock Protein 60 (HSP60) detection by QCM Biosensor and Antibody Covered Gold Nanoparticles
Original language description
Heat Shock Protein 60 (HSP60) is a 60 kDa weighting chaperonin that is an evolutionary conserved protein occurring in a wide number of organisms. It can serve as a plasma or blood serum biomarker of serious pathologies including cancer. In this work, quartz crystal microbalance (QCM) biosensor was constructed as a tool for a simple measurement of HSP 60 level. The QCMs were covered with an antibody specific to HSP60 and the antibody modified gold nanoparticles were also prepared. The assay was based of sandwich forming with captured HSP60, presence of HSP60 caused decrease of QCM oscillation frequency. The described assay exerted good sensitivity to HSP60, limit of detection for the assay was equal to 83 pg/ml for a sample with volume 5 mu l, one assay cycle was finished within 90 minutes. The assay well correlated to standard Enzyme-Linked ImmunoSorbent Assay (ELISA) with coefficient of determination 0.985 and was not sensitive to interference by selected proteins (HSP70, albumin, immunoglobulins). In a conclusion, the biosensor appears to be a reliable tool for the detection of HSP60, the achieved specifications make it promising for a routine application.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Electrochemical Science
ISSN
1452-3981
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
5
Country of publishing house
RS - THE REPUBLIC OF SERBIA
Number of pages
10
Pages from-to
210512
UT code for WoS article
000661483700012
EID of the result in the Scopus database
2-s2.0-85104728502