The use of molecular-genetic methods and bioinformatics tools to identify the representatives of the order Coleoptera occurring on decaying cadavers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919588" target="_blank" >RIV/62156489:43210/20:43919588 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The use of molecular-genetic methods and bioinformatics tools to identify the representatives of the order Coleoptera occurring on decaying cadavers
Original language description
The process of gradual settlement of the dead body by necrophagous insects plays an important role in forensic practice, especially when determining the time of death. This work deals with the decomposition of dead body of vertebrates by forensically important representatives from the order of Coleoptera in the Czech Republic. Experimental objects for our purposes were domestic pig and chicken. The research was realized from November 2017 to March 2019. During the study samples of larvae and adults were collected from the dead bodies and subsequently identified molecular-genetic methods. For research purposes was necessary to isolate the genomic DNA, amplify the fragment of cytochrome c oxidase I (COI) gene by the PCR method and sequencing this fragment DNA. The BLAST and BOLD databases showed the same outputs and based on the results the individuals were classified into individual families of the Coleoptera order.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
435-438
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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