Barcoding analysis for identification of insect species on decaying remains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43924753" target="_blank" >RIV/62156489:43210/23:43924753 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/23:43924753
Result on the web
<a href="https://doi.org/10.15414/afz.2023.26.04.347-353" target="_blank" >https://doi.org/10.15414/afz.2023.26.04.347-353</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15414/afz.2023.26.04.347-353" target="_blank" >10.15414/afz.2023.26.04.347-353</a>
Alternative languages
Result language
angličtina
Original language name
Barcoding analysis for identification of insect species on decaying remains
Original language description
Identifying necrophagous individuals on a dead body is most often associated with forensic practice and the field of forensic entomology. Insects are found on a dead body depending on climatic conditions, with the seasons naturally accelerating the decomposition of tissues and successive waves gradually reducing the amount of soft tissue remaining. The aim of this work was to compare the composition of the necrophagous invertebrate species occurring on different carcasses (Gallus gallus vs Sus scrofa f. domestica). A second objective was to evaluate which of the identification tools comparing COI gene sequences is currently more appropriate for barcoding and whether there are significant differences in the results of the BOLD and BLAST identification tools between the samples examined. Molecular determination was done using Sanger sequencing of the COI fragment of mitochondrial DNA. In total, 96 individuals and 13 species were identified in the Gallus gallus carcass and 50 individuals and 17 species on the Sus scrofa (f. domestica) carcass. Calliphora vomitoria was determined as the most abundant species in both corpses'remains. The reliability of the BOLD and BLAST identification methods is comparably high.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10616 - Entomology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Fytotechnica et Zootechnica
ISSN
1335-258X
e-ISSN
1336-9245
Volume of the periodical
26
Issue of the periodical within the volume
4
Country of publishing house
SK - SLOVAKIA
Number of pages
7
Pages from-to
347-353
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85182876668