Mettalothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919727" target="_blank" >RIV/62156489:43210/21:43919727 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00121981
Result on the web
<a href="https://doi.org/10.37904/nanocon.2020.3749" target="_blank" >https://doi.org/10.37904/nanocon.2020.3749</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3749" target="_blank" >10.37904/nanocon.2020.3749</a>
Alternative languages
Result language
angličtina
Original language name
Mettalothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry
Original language description
In this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICPMS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON 2020: Conference Proceedings
ISBN
978-80-87294-98-7
ISSN
2694-930X
e-ISSN
2694-930X
Number of pages
5
Pages from-to
385-389
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 21, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000664505500065