Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00574401" target="_blank" >RIV/67985904:_____/23:00574401 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00132618 RIV/62156489:43210/23:43923773

Result on the web

<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=5452124316" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=5452124316</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d3ja00065f" target="_blank" >10.1039/d3ja00065f</a>

Result language

angličtina

Original language name

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Original language description

Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu,M) was 63.3% (46.5 & mu,M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Analytical Atomic Spectrometry

ISSN

0267-9477

e-ISSN

1364-5544

Volume of the periodical

38

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1618-1625

UT code for WoS article

001022916300001

EID of the result in the Scopus database

2-s2.0-85165440787