Molecularly imprinted polymer nanoparticles as potential tool for immunohistochemistry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919820" target="_blank" >RIV/62156489:43210/21:43919820 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.37904/nanocon.2020.3751" target="_blank" >https://doi.org/10.37904/nanocon.2020.3751</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3751" target="_blank" >10.37904/nanocon.2020.3751</a>
Alternative languages
Result language
angličtina
Original language name
Molecularly imprinted polymer nanoparticles as potential tool for immunohistochemistry
Original language description
Immunohistochemistry (IHC) is a powerful technique enabling visualization/detection of cellular components (e.g. overexpressed proteins) in tissue samples and thus ensuring timely recognition of a wide range of diseases. Nowadays, antibodies labelled by different kinds of sensing probes are routinely used for selective recognition of IHC markers. However, antibodies as bio-macromolecules have a number of drawbacks including physical and (bio) chemical instability or large production cost. Therefore, the design and synthesis of artificial antibodies that are able to bind a target molecule with similar affinity and specificity have become a long-term goal in a range of scientific branches. In this work, molecularly imprinted polymer nanoparticles (MIPs), as antibody mimics, were used as IHC agents. Specifically, polydopamine MIP nanoparticles doped with metal ions and selective for metallothionein as potential IHC marker were developed and combined with laser ablation ICP-MS. This novel approach can significantly enhance the abilities of IHC analysis and solve issues connected with the low stability of antibodies.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON 2020: Conference Proceedings
ISBN
978-80-87294-98-7
ISSN
2694-930X
e-ISSN
2694-930X
Number of pages
5
Pages from-to
330-334
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 21, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000664505500056