Evaluation of stallion sperm concentration by two different methods and its influence on sperm motility assessment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921084" target="_blank" >RIV/62156489:43210/21:43921084 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.15414/afz.2021.24.04.293-296" target="_blank" >https://doi.org/10.15414/afz.2021.24.04.293-296</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/AFZ.2021.24.04.293-296" target="_blank" >10.15414/AFZ.2021.24.04.293-296</a>

Result language

angličtina

Original language name

Evaluation of stallion sperm concentration by two different methods and its influence on sperm motility assessment

Original language description

The aim of this study was to compare two methods for determining the concentration of sperm in stallion ejaculate and to evaluate the influence of different levels of concentration on the determination of sperm motility using an objective method. The total number of evaluated samples was 123. For the experiment, 14 stallions of different breeds housed in the Tlumačov Provincial Stud Farm were used. Sperm concentration was assessed by the hemocytometric method using a Bürker chamber and the automatic method using the Sperm Class Analyzer(R). The SCA system was used to evaluate sperm motility. The samples were divided into 6 groups with sperm concentrations of 50, 100, 150, 200, 250 and 300.106 sperm ml-1. The results were statistically evaluated by Tukey&apos;s HSD test. There were statistically significant differences in the determination of sperm concentrations between the hemocytometric method and the SCA system in samples with concentration exceeding 100.106 sperm ml-1. Sperm motility increased in samples with higher concentration, however the effect of sperm concentration on motility parameters has not been statistically significant. The results of this study indicate that samples with sperm concentration higher than 100.106 sperm ml-1 reduce the accuracy of the SCA system evaluation and these samples need to be diluted to concentration LESS-THAN OR EQUAL TO100.106 sperm ml-1 for more accurate evaluation.

Czech name

—

Czech description

—

Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Fytotechnica et Zootechnica

ISSN

1335-258X

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

4

Country of publishing house

SK - SLOVAKIA

Number of pages

4

Pages from-to

293-296

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85123091497