Exploring the pH-triggerable structure of siRNA-carrying liposomal nanoparticles as tools for treatment of hepatitis B
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921644" target="_blank" >RIV/62156489:43210/21:43921644 - isvavai.cz</a>
Result on the web
<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Exploring the pH-triggerable structure of siRNA-carrying liposomal nanoparticles as tools for treatment of hepatitis B
Original language description
Gene therapy using small interfering RNA (siRNA) molecules provides silencing of undesirable genes on mRNA translational level, which eliminates potential risks related to interactions with genomic DNA of the cell. However, due to their instability, these siRNA molecules must be delivered to the site of action via sophisticated delivery system. In this research work, we opted for liposomal vectors modified with polyethylene glycol chains attached by a pH-sensitive linkage. These liposomal nanoparticles were monitored in different buffer and pH conditions to verify some of their physicochemical properties required for an efficient siRNA delivery. Results from light scattering methods and cryo-transmission electron microscopy revealed that higher ionic strength environment reduced the diameter and zeta potential of the nanoparticles and that mildly acidic pH (6.0) caused structural changes leading to aggregation of the nanoparticles. These findings predetermine their favourable behaviour in biological systems.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2021: Proceedings of International PhD Students Conference
ISBN
978-80-7509-821-4
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
454-457
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 10, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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