Cross-linked-Pd0 polyethyleneimine catalyst for bioorthogonal chemistry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921841" target="_blank" >RIV/62156489:43210/21:43921841 - isvavai.cz</a>
Result on the web
<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Cross-linked-Pd0 polyethyleneimine catalyst for bioorthogonal chemistry
Original language description
Bioorthogonal therapy represents a promising tool for controlled pro-drug activation. However, efficient catalysis in the intracellular environment mediated by organometallic compounds still poses a great challenge. Herein, we describe the preparation of a palladium-trapped cross-linked catalyst. Polycationic polymer polyethyleneimine (PEI), an efficient transfection agent, was used as a carrier for palladium catalyst. To prevent palladium leaching and undesired palladium inactivation, PEI polymer with nested palladium catalyst was cross-linked with phosphine compound tetrakis(hydroxymethyl)phosphine chloride (THPC). The catalytic activity of cross-linked-Pd-PEI catalyst was assessed by the activation of a precursor fluorescent probe. The prepared catalyst exhibited catalytic activity in cell-free conditions in a PBS environment, as well as in a cell culture medium supplemented with FBS, which simulated the cellular environment.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2021: Proceedings of International PhD Students Conference
ISBN
978-80-7509-821-4
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
469-474
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 10, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—