Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923527" target="_blank" >RIV/62156489:43210/23:43923527 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.psj.2023.102807" target="_blank" >https://doi.org/10.1016/j.psj.2023.102807</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.psj.2023.102807" target="_blank" >10.1016/j.psj.2023.102807</a>
Alternative languages
Result language
angličtina
Original language name
Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation
Original language description
Mesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSCs of endangered Oravka chicken breed. MSCs was obtained from compact bones of the femur and tibiotarsus. MSCs were spindle-shaped and were able to differentiate into osteo-, adipo-, and chondrocytes under the specific differentiation conditions. Furthermore, MSCs were positive for surface markers such as CD29, CD44, CD73, CD90, CD105, CD146 and negative for CD34CD45 by flow cytometry. Moreover, MSCs demonstrated high positivity of "stemness" markers aldehyde dehydrogenase, alkaline phosphatase as well as for intracellular markers vimentin, desmin, α-SMA. Subsequently, MSCs were cryopreserved using 10% dimethyl sulfoxide in liquid nitrogen. Based on the results from the viability, phenotype and ultrastructure assessment we can concluded that the MSCs were not negatively affected by the cryopreservation. Finally, MSCs of endangered Oravka chicken breed were successfully stored in animal gene bank, thus making them a valuable genetic resource.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Poultry Science
ISSN
0032-5791
e-ISSN
1525-3171
Volume of the periodical
102
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
102807
UT code for WoS article
001056026700001
EID of the result in the Scopus database
2-s2.0-85161286501