Sperm quality of Zemplin Rabbit and Liptov Bold-Spotted Rabbit breeds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43924059" target="_blank" >RIV/62156489:43210/23:43924059 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.59913/dagr.2023.12358" target="_blank" >https://doi.org/10.59913/dagr.2023.12358</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.59913/dagr.2023.12358" target="_blank" >10.59913/dagr.2023.12358</a>

Result language

angličtina

Original language name

Sperm quality of Zemplin Rabbit and Liptov Bold-Spotted Rabbit breeds

Original language description

Zemplin Rabbit and Liptov Bold-Spotted Rabbit are Slovak national breeds. Our aim was to characterize the sperm quality of these two breeds, as these characteristics are important for artificial insemination and cryopreservation as biodiversity conservation tools. For this evaluation, sperm samples of sexually mature Zemplin Rabbit (ZR) males (n = 6) and Liptov Bold-Spotted Rabbits (LR) (n = 4) were used. According to progressive motility (PM) data (CASA), samples were divided into two groups: A (&gt;30% PM) and B (&lt;30% PM). In addition to PM (ZR-A: 48.6+-3.8%, ZR-B: 16+-3.2%, LR-A: 35.38+-2.6%, LR-B: 4+-2.2%), total motility (TM) (ZR-A: 69.1+-4.1%, ZR-B: 35.5+-4.1%, LR-A: 59.1+-3%, LR-B: 26.9+-4.1%) and morphological abnormalities (ZR-A: 29.7+-1.2%, ZR-B: 40+-4%, LR-A: 34.3+-4.3%, LR-B: 48.3+-4.7%) were also assessed using the CASA system. The proportion of dead/live, apoptotic and oxidative damaged spermatozoa (Spz) was assessed by flow cytometry using fluorescent dyes: SYBR-14, Sytox Green, Yo-Pro-1 and CellROX Green, respectively. The results of flow cytometry correspond to the values of motility and morphometry. Sperm with PM less than 30% does not show proper quality values, while the sperm with PM higher than 30% is suitable for further analysis and use.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Danubian Animal Genetic Resources

ISSN

2498-5910

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

2

Country of publishing house

HU - HUNGARY

Number of pages

8

Pages from-to

65-72

UT code for WoS article

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EID of the result in the Scopus database

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