High percentage of morphological defects in sperm of the Zemplin rabbit breed: A major obstacle to fertility?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F24%3A43925292" target="_blank" >RIV/62156489:43210/24:43925292 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.17221/29/2024-CJAS" target="_blank" >https://doi.org/10.17221/29/2024-CJAS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/29/2024-CJAS" target="_blank" >10.17221/29/2024-CJAS</a>

Result language

angličtina

Original language name

High percentage of morphological defects in sperm of the Zemplin rabbit breed: A major obstacle to fertility?

Original language description

This research investigates the high prevalence of morphological defects in the sperm of a particular rabbit breed, namely the Zemplin rabbit (ZR). We tried to uncover the possible causes and consequences of the observed abnormalities. Ejaculate samples from ten adult males were divided into two qualitative groups according to the motility parameters revealed by CASA (computer-assisted sperm analysis). Group A contained samples with total motility (TM) above 50% and progressive motility (PM) above 30%. Group B contained samples with TM and PM below these values. Viability (SYBR-14/DRAQ5TM and SYTOXTM Green/DRAQ5TM staining) and apoptosis (YO-PRO TM-1/ DRAQ5 TM stainig) analyses through flow cytometry served as an additional insight into the characteristics of the qualitative groups. In both cases, we noted significant differences (P &lt; 0.05) between groups which corresponded to motility values. Both groups showed an increased incidence of abnormal spermatozoa, and we did not observe any significant differences between them. Simultaneously, we assessed the practical implications of these sperm defects on artificial insemination (AI), and also, we did not notice any significant differences in these parameters. The use of transmission electron microscopy enhanced our understanding of the morphological defects at the ultrastructural level. In summary, the study provides an understanding of the qualitative differences in rabbit semen, highlighting variations in quality parameters. While these differences exist, they may not necessarily translate into significant variations in AI outcomes. Our study shows higher values of cytoplasmic droplets in ZR semen, but it also shows that these conditions do not necessarily mean a fundamental obstacle to fertility in the case of AI.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

1805-9309

Volume of the periodical

69

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

191-199

UT code for WoS article

001235880400001

EID of the result in the Scopus database

2-s2.0-85195851482