Metabolic adaptations of Escherichia coli to extended zinc exposure: Insights into tricarboxylic acid cycle and trehalose synthesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F24%3A43925637" target="_blank" >RIV/62156489:43210/24:43925637 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1186/s12866-024-03463-6" target="_blank" >https://doi.org/10.1186/s12866-024-03463-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12866-024-03463-6" target="_blank" >10.1186/s12866-024-03463-6</a>

Result language

angličtina

Original language name

Metabolic adaptations of Escherichia coli to extended zinc exposure: Insights into tricarboxylic acid cycle and trehalose synthesis

Original language description

Balanced bacterial metabolism is essential for cell homeostasis and growth and can be impacted by various stress factors. In particular, bacteria exposed to metals, including the nanoparticle form, can significantly alter their metabolic processes. It is known that the extensive and intensive use of food and feed supplements, including zinc, in human and animal nutrition alters the intestinal microbiota and this may negatively impact the health of the host. This study examines the effects of zinc (zinc oxide and zinc oxide nanoparticles) on key metabolic pathways of Escherichia coli. Transcriptomic and proteomic analyses along with quantification of intermediates of tricarboxylic acid (TCA) were employed to monitor and study the bacterial responses. Multi-omics analysis revealed that extended zinc exposure induced mainly oxidative stress and elevated expression/production of enzymes of carbohydrate metabolism, especially enzymes for synthesis of trehalose. After the zinc withdrawal, E. coli metabolism returned to a baseline state. These findings shed light on the alteration of TCA and on importance of trehalose synthesis in metal-induced stress and its broader implications for bacterial metabolism and defense and consequently for the balance and health of the human and animal microbiome.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multidisciplinary research to increase application potential of nanomaterials in agricultural practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Microbiology

ISSN

1471-2180

e-ISSN

1471-2180

Volume of the periodical

24

Issue of the periodical within the volume

1 October

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

384

UT code for WoS article

001326691200002

EID of the result in the Scopus database

2-s2.0-85205528055