Diversity and impact of single-stranded RNA viruses in Czech Heterobasidion populations
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F24%3A43925663" target="_blank" >RIV/62156489:43210/24:43925663 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/24:43925663
Result on the web
<a href="https://doi.org/10.1128/msystems.00506-24" target="_blank" >https://doi.org/10.1128/msystems.00506-24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/msystems.00506-24" target="_blank" >10.1128/msystems.00506-24</a>
Alternative languages
Result language
angličtina
Original language name
Diversity and impact of single-stranded RNA viruses in Czech Heterobasidion populations
Original language description
Heterobasidion annosum sensu lato comprises some of the most devastating pathogens of conifers. Exploring virocontrol as a potential strategy to mitigate economic losses caused by these fungi holds promise for the future. In this study, we conducted a comprehensive screening for viruses in 98 H. annosum s.l. specimens from different regions of Czechia aiming to identify viruses inducing hypovirulence. Initial examination for dsRNA presence was followed by RNA-seq analyses using pooled RNA libraries constructed from H. annosum and Heterobasidion parviporum, with diverse bioinformatic pipelines employed for virus discovery. Our study uncovered 25 distinct ssRNA viruses, including two ourmia-like viruses, one mitovirus, one fusarivirus, one tobamo-like virus, one cogu-like virus, one bisegmented narna-like virus and one segment of another narna-like virus, and 17 ambi-like viruses, for which hairpin and hammerhead ribozymes were detected. Coinfections of up to 10 viruses were observed in six Heterobasidion isolates, whereas another six harbored a single virus. Seventy-three percent of the isolates analyzed by RNA-seq were virus-free. These findings show that the virome of Heterobasidion populations in Czechia is highly diverse and differs from that in the boreal region. We further investigated the host effects of certain identified viruses through comparisons of the mycelial growth rate and proteomic analyses and found that certain tested viruses caused growth reductions of up to 22% and significant alterations in the host proteome profile. Their intraspecific transmission rates ranged from 0% to 33%. Further studies are needed to fully understand the biocontrol potential of these viruses in planta.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40102 - Forestry
Result continuities
Project
—
Continuities
O - Projekt operacniho programu
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
mSystems
ISSN
2379-5077
e-ISSN
2379-5077
Volume of the periodical
9
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
27
Pages from-to
"e00506"-24
UT code for WoS article
001314605300011
EID of the result in the Scopus database
2-s2.0-85207598596