ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F25%3A43927564" target="_blank" >RIV/62156489:43210/25:43927564 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00142956
Result on the web
<a href="https://doi.org/10.1016/j.genrep.2025.102352" target="_blank" >https://doi.org/10.1016/j.genrep.2025.102352</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.genrep.2025.102352" target="_blank" >10.1016/j.genrep.2025.102352</a>
Alternative languages
Result language
angličtina
Original language name
ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles
Original language description
The application of zinc oxide nanoparticles (ZnO NPs) in agriculture and plant research is a topic of significant debate. In this study, we explored the molecular responses of Arabidopsis thaliana (Columbia (Col-0) ecotype) to ZnO NP exposure, with a primary focus on miRNA expression and metabolite profiles, providing new insights into the mechanisms underlying plant responses. ZnO NPs were applied at three concentrations (8, 40, 80 mg.kgMINUS SIGN 1) in peat tablets (Jiffy(R)). Our results reveal dose-dependent negative effects on plant phenotypic traits, particularly reductions in growth and chlorophyll content. Notably, ZnO NPs caused significant disruptions in metal homeostasis, with elevated zinc accumulation and altered uptake of manganese, iron, and calcium. Gene expression analysis of antioxidant defense responses demonstrated concentration-specific regulation, indicating a shift towards more specialized antioxidants under ZnO NP stress. Specifically, the lower concentrations (8 and 40 mg.kgMINUS SIGN 1) led to the overexpression of genes related to H2O2 scavenging and glutathione synthesis, while the 80 mg.kgMINUS SIGN 1 concentration induced the upregulation of genes associated with superoxide scavenging, highlighting a dose-dependent shift in oxidative stress response mechanisms. These molecular changes were accompanied by significant alterations in miRNA expression and metabolite profiles, in a non-linear and dose-dependent manner. A significant role was observed for the miR156-SPL regulatory module at the 40 and 80 mg.kgMINUS SIGN 1 concentrations. Furthermore, proline (approx. 20 % increase, 8, 80 mg.kgMINUS SIGN 1), taurine (16 %, 80 mg.kgMINUS SIGN 1), fumaric acid (31 %, 40 mg.kgMINUS SIGN 1), and glutamic acid (50 %, 8 mg.kgMINUS SIGN 1) were implicated in the plant's adaptive response to ZnO NPs, contributing to antioxidant defense and metal-binding mechanisms. Together, these results highlight that ZnO NP exposure elicits complex and multi-layered adaptive mechanisms involving the interplay of elemental stress, antioxidant responses, miRNA regulation, and metabolic adjustments.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Gene Reports
ISSN
2452-0144
e-ISSN
2452-0144
Volume of the periodical
41
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
102352
UT code for WoS article
001600047800001
EID of the result in the Scopus database
2-s2.0-105018504804