Flow cytometry analysis and PCR with chromosome-specific primers of chromosome suspension of Norway spruce

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F99%3A21100013" target="_blank" >RIV/62156489:43210/99:21100013 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Flow cytometry analysis and PCR with chromosome-specific primers of chromosome suspension of Norway spruce
Original language description
Flow cytometric analysis of suspension of isolated chromosomes enables classification of chromosmes in the form of histograms (called flow-karyotypes) based on biochemical features such as their relative DNA content, base content, protein content, and various morphological parameters. Flow karyotyping has found applications in monitoring karyotype changes, for the detection of aberrant chromosomes, and for mutagenicity testing. A high-yield method for isolation of Norway spruce chromosomes in suspension, their analysis and sorting using flow cytometry is described. To accumulate meristem root tips cells at metaphase, actively growing roots were subjected treatments with 0,05 % colchicine for 24h (Überall et al. 1997).
Czech name
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Czech description
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Project
<a href="/en/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Novel approaches for effective analysis and mapping of genomes in cultivated plants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
International Plant Physiology Congress of Ph.D. Students and Young Scientists
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Comenius University
Place of publication
Bratislava, Slovak Republic
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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