Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F11%3A43908604" target="_blank" >RIV/62156489:43410/11:43908604 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.cryobiol.2011.04.004" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2011.04.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2011.04.004" target="_blank" >10.1016/j.cryobiol.2011.04.004</a>

Result language

angličtina

Original language name

Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Original language description

In coniferous species, including Greek fir (Abies cephalonica Loud), the involvement of somatic embryo plants in breeding and reforestation programs is dependent on the success of long-term cryostorage of embryogenic cultures during clonal field testing.In the present study on Greek fir, we assayed the recovery, morphological characteristics and genetic fidelity of embryogenic cell lines 6 and 8 during proliferation and maturation after long-term cryostorage. Our results indicate successful recovery ofboth cell lines after 6 years in cryostorage. In the maturation phase, both cell lines were capable of producing somatic embryos although some differences were detected among experiments. However, these changes were more dependent on the differences inthe components of the maturation media or in the experimental set-up than on the long-term cryostorage. During both proliferation and maturation phases, the morphological fidelity of the embryogenic cultures as well as of the somatic embr

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GK - Forestry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cryobiology

ISSN

0011-2240

e-ISSN

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Volume of the periodical

63

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

17-25

UT code for WoS article

000292020800004

EID of the result in the Scopus database

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