Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F13%3A43908656" target="_blank" >RIV/62156489:43410/13:43908656 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11627-013-9512-1" target="_blank" >http://dx.doi.org/10.1007/s11627-013-9512-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11627-013-9512-1" target="_blank" >10.1007/s11627-013-9512-1</a>
Alternative languages
Result language
angličtina
Original language name
Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
Original language description
Somatic embryogenesis of Abies alba (Mill.) has significant potential to become an effective method for vegetative propagation of this species. To induce somatic embryogenesis in A. alba, the influence of the mother tree, sampling dates, and cold treatment storage of cones were examined. The initiation frequencies ranged from 1.7% to 16.6%. The sampling date and cone storage, but not the mother tree, had a significant effect on the initiation of embryogenic cultures. Storage of embryogenic cell lines was tested through cryopreservation for 6 yr. Four out of 12 cryostored embryogenic cell lines recovered, and the regeneration of cotyledonary embryos was obtained with two cell lines. The ability of embryogenic cell masses to produce somatic embryos and the mean number of cotyledonary embryos were higher when the maturation protocol was based on embryogenic suspensions dispersed on filter paper. The properly developed germinants were obtained only from maturation media where 32 mu M absci
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GK - Forestry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
In Vitro Cellular & Developmental Biology-Plant
ISSN
1054-5476
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
560-571
UT code for WoS article
000326103900008
EID of the result in the Scopus database
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