Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F22%3A43921792" target="_blank" >RIV/62156489:43410/22:43921792 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-0716-2517-0_2" target="_blank" >https://doi.org/10.1007/978-1-0716-2517-0_2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-2517-0_2" target="_blank" >10.1007/978-1-0716-2517-0_2</a>
Alternative languages
Result language
angličtina
Original language name
Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests
Original language description
Phytophthora species can be found in multiple substrates. Due to dormancy of resting structures and presence of faster-growing antagonists, direct isolation of Phytophthora can be difficult to achieve, and indirect baiting methods often reach higher isolation frequencies. In this chapter, different methodologies are described for sampling and for the successful isolation of Phytophthora species from natural ecosystems. Sampling methods for soil, roots, bark cankers, and waterbodies are described. Agar recipes and guidance on the selection of suitable tissue to perform direct isolations are provided. A range of different baiting techniques are described for isolation of Phytophthora from different substrates. Purification methods to obtain clean and non-mixed cultures and conservation methods for pure cultures are shown. Two pathogenicity test methods for soil infestation and for under-bark inoculation, respectively, are described in detail.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
40102 - Forestry
Result continuities
Project
<a href="/en/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Phytophthora Research Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Plant Pathology: Methods and Protocols
ISBN
978-1-07-162516-3
Number of pages of the result
37
Pages from-to
13-49
Number of pages of the book
497
Publisher name
Humana Press Inc./Springer
Place of publication
Totowa
UT code for WoS chapter
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