Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F25%3A43927126" target="_blank" >RIV/62156489:43410/25:43927126 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00705-025-06313-6" target="_blank" >https://doi.org/10.1007/s00705-025-06313-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00705-025-06313-6" target="_blank" >10.1007/s00705-025-06313-6</a>
Alternative languages
Result language
angličtina
Original language name
Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea
Original language description
Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named "Diplodia sapinea flexi-like virus 1" (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start codons, encode putative proteins with unknown functions. Phylogenetic analysis placed DsFLV1 in a distinct clade within the family Deltaflexiviridae, close to Erysiphe necator-associated flexivirus 2. This is the first report of a positive-sense single-stranded RNA virus infecting D. sapinea.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Virology
ISSN
0304-8608
e-ISSN
1432-8798
Volume of the periodical
170
Issue of the periodical within the volume
6
Country of publishing house
AT - AUSTRIA
Number of pages
4
Pages from-to
131
UT code for WoS article
001489449100004
EID of the result in the Scopus database
2-s2.0-105005274509