SSR analyses within Prunus genus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F04%3A00003557" target="_blank" >RIV/62156489:43510/04:00003557 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

SSR analyses within Prunus genus

Original language description

SSR polymorphisms are extensively used as genetic markers. They are generally codominant, highly polymorphic, they have high eficiency in a species with low variability such as peach and can be detected with a simple PCR procedure. Consequently SSR wereused for study of the genetic diversity in Prunus genus. The 9 known SSR pair of primers nominated by other autors were applied to 17 Prunus cultivars. SSR analyses were performed with the thermal cycler program by the use of touchdown protocol for annealing The electrophoretic spectrum of the obtained bands on the agarose MetaPhor were similar to the spectrum on polyacrylamide (PAGE). The gel on the Metaphor was visualised by ethidium bromide staining, the polyacrylamide gel by the SYBR Gold. The primers distinguished few a homozygous and heterozygous genotypes in given locus within all analysed cultivars. The given sizes of the SSR products were compared with the resulting product sizes in work of Testolin et al. (2000). These results

Czech name

SSR analyses within Prunus genus

Czech description

SSR polymorphisms are extensively used as genetic markers. They are generally codominant, highly polymorphic, they have high eficiency in a species with low variability such as peach and can be detected with a simple PCR procedure. Consequently SSR wereused for study of the genetic diversity in Prunus genus. The 9 known SSR pair of primers nominated by other autors were applied to 17 Prunus cultivars. SSR analyses were performed with the thermal cycler program by the use of touchdown protocol for annealing The electrophoretic spectrum of the obtained bands on the agarose MetaPhor were similar to the spectrum on polyacrylamide (PAGE). The gel on the Metaphor was visualised by ethidium bromide staining, the polyacrylamide gel by the SYBR Gold. The primers distinguished few a homozygous and heterozygous genotypes in given locus within all analysed cultivars. The given sizes of the SSR products were compared with the resulting product sizes in work of Testolin et al. (2000). These results

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GP521%2F03%2FP133" target="_blank" >GP521/03/P133: Early selection of apricot genotypes on resistance against Sharka (PPV) using molecular genetic methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

International Conference on Horticulture Post-graduate (Ph.D.) Study System and Conditions in Europe

ISBN

80-7157-801-0

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

48-48

Publisher name

Mendelova zemědělská a lesnická univerzita v Brně

Place of publication

Brno

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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