Comparison of Methods of Isolating DNA for ESFY Phytoplasma Detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F09%3A00170187" target="_blank" >RIV/62156489:43510/09:00170187 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Comparison of Methods of Isolating DNA for ESFY Phytoplasma Detection
Original language description
'Candidatus phytoplasma prunorum', (European stone fruit yellows phytoplasma - ESFY), is nowadays one of the most important diseases belonging to the list of organisms for which quarantine is required, and is a long-standing problem in apricot and peachorchards in the Czech Republic. Its detection and diagnosis is based on molecular methods, especially "nested PCR". There are various methods for the isolation of DNA from leaf-stalks and the phloem (from stalk or roots), and four different methods weretested: Lee et al. (1991), two commercial DNA isolation kits (DNeasy Plant Maxi Kit and NucleoSpin; Plant XL) and, as a comparison, the method described by Ahrens and Seemüller (1992). For the nested PCR detection of ESFY we used non-specific primers like R16F1/R16R0 (out-primers) and fU5/rU3 (in-primers). The results demonstrate that none of the methods used for the DNA isolation and subsequent PCR reactions provide the consistent quality and quantity of DNA which is necessary for ESFY
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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