Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F09%3A00170204" target="_blank" >RIV/62156489:43510/09:00170204 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimization of the Preparation of ds cDNA from Flower Buds of Apricot for the cDNA-AFLP Analysis
Original language description
The aim of the work was to develop the preparation of ds cDNA from flower buds of four apricot varieties ('Sundrop', 'Stark Early Orange', 'Vestar' and 'Betinka') so that it would be as suitable as possible for the cDNA-AFLP analysis. The sampled tissueswere preserved using a commercial agent RNAlater Soln. (Ambion). The RNA from the chosen samples was isolated using a commercial isolation kit supplied by Sigma (SpectrumTM Plant Total RNA Kit), which support the isolation of sufficient amounts of highquality RNA from these highly complicated tissues. Transcription from the entire RNA onto the ds cDNA was achieved using the MINT cDNA synthesis kit from Evrogen. The cDNA-AFLP analysis was carried out according to an amended procedure by BACHEM et al. (1998) and the samples obtained from selective amplification were analysed on genetic analyser ABI-PRISM 310. The results obtained by another protocol (include silica extraction of RNA, GenElute mRNA Miniprep Kit and others) were compared
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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