Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F14%3A00214101" target="_blank" >RIV/62156489:43510/14:00214101 - isvavai.cz</a>
Result on the web
<a href="http://theglobaljournals.com/ijsr/issues.php?m=March&y=2014&id=23" target="_blank" >http://theglobaljournals.com/ijsr/issues.php?m=March&y=2014&id=23</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Evaluation of Three Approaches For The Measurement of Rna Integrity, Concentration and Purity in Tissues of Apricot Flower Buds
Original language description
The RNA quantity and quality are basic factors for successful studies of gene expression. On absorbance and fluorescence based measurements are routinely used techniques for RNA measurement. A new technique for measurement of RNA is available with Agilent 2100 bioanalyzer in combination with the RNA 6000 Nano LabChip kit. This method offers in addition the advantage of assessing RNA integrity. Agilent 2100 Bioanalyzer is compared with UV spectrophotometry and fluorometry quantitation systems assessing the quantity, purity and RNA integrity. This study was focused on comparison of RNA isolated from apricot flower buds which were evaluated by mentioned methods. Obtained results show important differences in RNA which was isolated in 2008 (stored at -80 C) and RNA which was newly isolated from the same stored tissues in 2013. Study also compares techniques of mRNA quantity, quality and integrity evaluation.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Scientific Research
ISSN
2277-8179
e-ISSN
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Volume of the periodical
3
Issue of the periodical within the volume
3
Country of publishing house
IN - INDIA
Number of pages
4
Pages from-to
157-160
UT code for WoS article
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EID of the result in the Scopus database
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