All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F15%3A43907066" target="_blank" >RIV/62156489:43510/15:43907066 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf" target="_blank" >http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves

  • Original language description

    The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determinationof Xcc on the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three setsof primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv.campestris detection protocol were recommended - commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GE - Plant cultivation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LH12196" target="_blank" >LH12196: Development of crop stress metabolism and genomics methods in Czech Republic and China</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Acta Scientiarum Polonorum. Hortorum Cultus

  • ISSN

    1644-0692

  • e-ISSN

  • Volume of the periodical

    14

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    PL - POLAND

  • Number of pages

    10

  • Pages from-to

    141-150

  • UT code for WoS article

    000365962000010

  • EID of the result in the Scopus database

    2-s2.0-84949229042