Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F15%3A43907066" target="_blank" >RIV/62156489:43510/15:43907066 - isvavai.cz</a>
Result on the web
<a href="http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf" target="_blank" >http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves
Original language description
The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determinationof Xcc on the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three setsof primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv.campestris detection protocol were recommended - commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LH12196" target="_blank" >LH12196: Development of crop stress metabolism and genomics methods in Czech Republic and China</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Scientiarum Polonorum. Hortorum Cultus
ISSN
1644-0692
e-ISSN
—
Volume of the periodical
14
Issue of the periodical within the volume
6
Country of publishing house
PL - POLAND
Number of pages
10
Pages from-to
141-150
UT code for WoS article
000365962000010
EID of the result in the Scopus database
2-s2.0-84949229042