Detection of Xanthomonas campestris pv. campestris through a real-time PCR assay targeting the Zur gene and comparison with detection targeting the hrpF gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43916155" target="_blank" >RIV/62156489:43510/19:43916155 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s10658-019-01820-0" target="_blank" >https://doi.org/10.1007/s10658-019-01820-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10658-019-01820-0" target="_blank" >10.1007/s10658-019-01820-0</a>

Result language

angličtina

Original language name

Detection of Xanthomonas campestris pv. campestris through a real-time PCR assay targeting the Zur gene and comparison with detection targeting the hrpF gene

Original language description

Xanthomonas campestris pv. campestris (Xcc) is a seedborne bacterium that causes black rot of crucifers. A real-time PCR assay based on a dual-labeled hydrolysis TaqMan(R) probe has been developed for the rapid and sensitive detection of Xcc and related pathovars that affect mainly Brassicaceae crops and ornamentals. Primers were designed to specifically amplify a 152 bp fragment of the Zur gene from X. campestris. To confirm the specificity of the detection, primers targeting the Zur and hrpF genes were used for standard and real-time PCR with DNA samples from 13 Xcc strains, seven Xanthomonas species and pathovars and five different bacterial endophytes including Bacillus, Erwinia, Klebsiella, Pantoea and Pseudomonas, previously isolated from tissues of crucifers. PCR products amplified with Zur and hrpF primers were sequenced to assess the genetic diversity of these genes in the tested isolates. The real-time PCR protocol was optimized to allow the detection at the level of ten copies of Zur PCR fragment per one microliter of DNA. Although the real-time based on detection of Zur also detected X. campestris pvs raphani, armoraciae, incanae and a strain of X. hortorum pv. carotae, it improved the specificity in relation to the previously published hrpF based real-time method. A multiplex assay for Zur and hrpF genes further improved the specificity by excluding X. hortorum pv. carotae. Tests of brassica tissues and seeds artificially inoculated with Xcc showed that the real-time PCR based on detection of Zur is an efficient and robust assay.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Plant Pathology

ISSN

0929-1873

e-ISSN

—

Volume of the periodical

155

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

891-902

UT code for WoS article

000491550300015

EID of the result in the Scopus database

2-s2.0-85070294952