Usage of UV irradiation for nucleus destruction of Petunia hybrida

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912585" target="_blank" >RIV/62156489:43510/17:43912585 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Usage of UV irradiation for nucleus destruction of Petunia hybrida
Original language description
Production costs of F1 seeds of Petunia hybrida can be decreased by using sterile component. Cytoplasmic male sterility (CMS), located in mitochondria, is transferred either by back crossing or by protoplast fusion. Asymmetric protoplast fusion represents the most effective way. Protoplast nucleus of one component (donor of CMS) must be destroyed by UV radiation. Protoplast cytoplasm of the second component must by destroyed by metabolic inhibitors. Asymmetrically fused protoplast possesses CMS from donor and rest of the features of fertile component. The aim of this experiment was to determine the amount of UV radiation (in seconds) needed for destruction of protoplast nucleus. Optimal UV radiation, based on results from 3 different Petunia hybrida genotypes is 510 µW/cm2 for a duration of 300 seconds.
Czech name
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Czech description
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Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Number of pages
5
Pages from-to
603-607
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500107

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