Evaluation of presence and concentration of ppv in rootstocks derived from prunus davidiana (carr.) Franch

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43915531" target="_blank" >RIV/62156489:43510/19:43915531 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.11118/actaun201967010121" target="_blank" >https://doi.org/10.11118/actaun201967010121</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201967010121" target="_blank" >10.11118/actaun201967010121</a>

Result language

angličtina

Original language name

Evaluation of presence and concentration of ppv in rootstocks derived from prunus davidiana (carr.) Franch

Original language description

Evaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016-2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF-305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method - semiquantitative DAS-ELISA test and molecular methods - RT-PCR, real-time RT-PCR and RT-LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS-ELISA test giving less positive samples than RT-PCR. The RT-LAMP and real-time RT-PCR methods were capable of revealing low concentrations of the virus even in symptom-free plants. The lowest PPV concentrations of all the four rootstocks were detected by real-time RT-PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QJ1510081" target="_blank" >QJ1510081: Innovation of the crucial techniques in fruit nursery production.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Volume of the periodical

67

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

121-131

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85062531174