High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43915562" target="_blank" >RIV/62156489:43510/19:43915562 - isvavai.cz</a>
Alternative codes found
RIV/00027006:_____/19:00005100
Result on the web
<a href="https://doi.org/10.1016/j.jviromet.2019.03.003" target="_blank" >https://doi.org/10.1016/j.jviromet.2019.03.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jviromet.2019.03.003" target="_blank" >10.1016/j.jviromet.2019.03.003</a>

Result language
angličtina
Original language name
High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy
Original language description
This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were sanitized using Ribavirin. Three years after sanitation, a small RNA sequencing method for virus detection, targeting 21, 22 and 24 nt-long viral small RNAs (vsRNAs), was used to analyze both the mother plant and the sanitized plants. The results showed that the mother plant was infected by the three mentioned viruses and additionally by two viroids - Hop stunt viroid and Grapevine yellow speckle viroid 1. After Ribavirin treatment, the plants contained only the two viroids, with the complete elimination of all the viruses previously present.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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