Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F06%3A00000666" target="_blank" >RIV/62157124:16170/06:00000666 - isvavai.cz</a>

Alternative codes found

RIV/61988987:17110/06:A1100VHX

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Original language description

Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isolates prepared by anaerobic cultivation was carried outby multiplex PCR. C. perfringens type A (37.9%) and type C (1.3%) were detected on 58 and 2 farms, respectively. The cpb2 gene was found in 79.3% strains of C. perfringens type A and in 100% strains of type C. An evaluation of the prevalence of positiveidentification of C. perfringens with the cpb2 gene shows that the microorganism was diagnosed on large farms with the basic herd of over 700 or 1 000 sows significantly more frequently. None of the isolates was positive as to the specific sequences ofthe enterotoxin-coding gene.

Czech name

Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Czech description

Out of the total of 153 farms under investigation that had been experiencing diarrhoea in suckling piglets, the presence of Clostridium perfringens was detected on 60 farms (39.2%). PCR typing of isolates prepared by anaerobic cultivation was carried outby multiplex PCR. C. perfringens type A (37.9%) and type C (1.3%) were detected on 58 and 2 farms, respectively. The cpb2 gene was found in 79.3% strains of C. perfringens type A and in 100% strains of type C. An evaluation of the prevalence of positiveidentification of C. perfringens with the cpb2 gene shows that the microorganism was diagnosed on large farms with the basic herd of over 700 or 1 000 sows significantly more frequently. None of the isolates was positive as to the specific sequences ofthe enterotoxin-coding gene.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

9

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

461-467

UT code for WoS article

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EID of the result in the Scopus database

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