Dynamics of caspase-3 inhibition in embryonic mouse limbs after developmentally and experimentally induced apoptosis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F13%3A43872474" target="_blank" >RIV/62157124:16170/13:43872474 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Dynamics of caspase-3 inhibition in embryonic mouse limbs after developmentally and experimentally induced apoptosis
Original language description
Caspases are key molecules of physiological cell death activated in proteolytic cascade and play a fundamental role during embryonic and postnatal development, particularly in apoptotic events. Caspase-3 is a central caspase of apoptosis, therefore, a common target for manipulations in research and development of anti-cancer therapies. Fluoromethylketones (FMK) are widely used for pharmacological inhibition of caspases, nevertheless, several questions related to inhibitor penetrability, stability and inhibition dynamics remain unclear. Mouse limb digitalization represents the most well known example of apoptosis. This work focuses on caspase-3 inhibition by FMK-inhibitor at the stage prior to the interdigital regression (E12). Two different ex vivo/invitro approaches, explants cultures and mesenchymal micromasses, were exploited to investigate two aspects. The effect of caspase-3 inhibition on limb digitalization was followed using front limb cultures. Inhibition dynamics was evaluate
Czech name
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Czech description
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Project
<a href="/en/project/GAP502%2F12%2F1285" target="_blank" >GAP502/12/1285: Apoptotic and non-apoptotic functions of caspases in hard tissue formation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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