Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876290" target="_blank" >RIV/62157124:16170/18:43876290 - isvavai.cz</a>
Alternative codes found
RIV/68081766:_____/18:00489268 RIV/60077344:_____/18:00498784 RIV/62157124:16810/18:43876290 RIV/00216208:11310/18:10382047
Result on the web
<a href="http://dx.doi.org/10.1038/s41598-018-24126-3" target="_blank" >http://dx.doi.org/10.1038/s41598-018-24126-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-24126-3" target="_blank" >10.1038/s41598-018-24126-3</a>
Alternative languages
Result language
angličtina
Original language name
Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species
Original language description
Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/GA15-05180S" target="_blank" >GA15-05180S: Genetic diversity and cross-transmissions of strongylid nematodes between African great apes and man</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
APR
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
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UT code for WoS article
000429785900073
EID of the result in the Scopus database
2-s2.0-85045440671