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Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876290" target="_blank" >RIV/62157124:16170/18:43876290 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081766:_____/18:00489268 RIV/60077344:_____/18:00498784 RIV/62157124:16810/18:43876290 RIV/00216208:11310/18:10382047

  • Result on the web

    <a href="http://dx.doi.org/10.1038/s41598-018-24126-3" target="_blank" >http://dx.doi.org/10.1038/s41598-018-24126-3</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41598-018-24126-3" target="_blank" >10.1038/s41598-018-24126-3</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species

  • Original language description

    Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

    <a href="/en/project/GA15-05180S" target="_blank" >GA15-05180S: Genetic diversity and cross-transmissions of strongylid nematodes between African great apes and man</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scientific Reports

  • ISSN

    2045-2322

  • e-ISSN

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    APR

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000429785900073

  • EID of the result in the Scopus database

    2-s2.0-85045440671