Properties of potentially probiotic Lactobacillus isolates from poultry intestines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F19%3A43877984" target="_blank" >RIV/62157124:16170/19:43877984 - isvavai.cz</a>
Result on the web
<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088010073.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088010073.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201988010073" target="_blank" >10.2754/avb201988010073</a>
Alternative languages
Result language
angličtina
Original language name
Properties of potentially probiotic Lactobacillus isolates from poultry intestines
Original language description
The most commonly used probiotic bacteria belong to the genus Lactobacillus, being regarded as beneficial for poultry health and production. However, commercial probiotics do not always ensure both expected effects. In order to improve the utility properties of new preparations, the selection of new probiotic candidates should be made on the basis of the performance of the species within the poultry digestive tract. The aim of this study was to isolate and identify lactobacilli from poultry intestines, and to select probiotic candidates for subsequent in vivo trials. Digesta from 18 poultry specimens were collected, serially diluted, plated onto Wilkins-Chalgren anaerobe agar supplemented with 30% of rumen fluid and onto De Man, Rogosa and Sharpe agar plates, and incubated at 37 degrees C for 48 h under anaerobic conditions. Isolated colonies were subjected to Gram staining and catalase reaction. They were then pre-identified using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectrometry (MS). Forty-four Lactobacillus strains belonging to 16 species were identified and subjected to evaluations of survival under simulated gastrointestinal conditions, autoaggregation and hydrophobicity. Most of the screened Lactobacillus reuteri strains as well as individual strains of L. acidophilus, L. amylovorus, L. gallinarum, L. ingluviei, L. johnsonii, L. oris, L. salivarius, L. saerimneri, and L. vaginalis showed high survival rates under gastrointestinal tract conditions and good surface properties. The results suggest their potential for further testing as probiotic candidates in in vivo trials.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/QJ1610219" target="_blank" >QJ1610219: Use of gut microbiota to increase natural resistance of meat type of chickens to bacterial infections</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
88
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
73-84
UT code for WoS article
000468126600010
EID of the result in the Scopus database
2-s2.0-85065499673