Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F23%3AN0000046" target="_blank" >RIV/26722861:_____/23:N0000046 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/23:43927567 RIV/60461373:22340/23:43927567

Result on the web

<a href="https://doi.org/10.3390/fermentation9110960" target="_blank" >https://doi.org/10.3390/fermentation9110960</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/fermentation9110960" target="_blank" >10.3390/fermentation9110960</a>

Result language

angličtina

Original language name

Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Original language description

Infant feces and breast milk are sources of lactic acid bacteria with interesting functional and technological properties. Therefore, we focused on the isolation and characterization of new potential probiotic strains from infant feces and breast milk. Nine strains were identified based on 16S rRNA sequences of Lacticaseibacillus rhamnosus, Limosilactobacillus reuteri, and Lactobacillus gasseri. Subsequently, these isolates were evaluated for their safety (antibiotic resistance, hemolytic, and enzymatic activity) and functional properties (gastrointestinal condition tolerance, hydrophobicity, auto-aggregation, adhesion to Caco-2/HT-29 cell lines, antimicrobial and antioxidant properties, and the production of exopolysaccharides). All the isolates were non-hemolytic and resistant to streptomycin, kanamycin, and vancomycin. They also demonstrated antimicrobial activity against five selected pathogens (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella sp., and Enterococcus faecalis) and the production of exopolysaccharides. Isolates Lacticaseibacillus rhamnosus S1F and S6C together with Lactobacillus gasseri M8C showed the highest ability to survive 3.0 g/L of bile salts and a good adhesion property, which is important for gastrointestinal tract (GIT) colonization or the inhibition of pathogens. Following in vivo characterization, three strains S1F and S6C from infant feces, and M6C from breast milk, showed probiotic properties with potential applications in the food industry

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fermentation

ISSN

2311-5637

e-ISSN

2311-5637

Volume of the periodical

9

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

960

UT code for WoS article

001123346600001

EID of the result in the Scopus database

2-s2.0-85178255993