CTX-M-producing Escherichia coli in pigs from a Czech farm during production cycle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F20%3A43878745" target="_blank" >RIV/62157124:16170/20:43878745 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/20:43878745 RIV/62157124:16810/20:43878745
Result on the web
<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13331" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/lam.13331</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/lam.13331" target="_blank" >10.1111/lam.13331</a>
Alternative languages
Result language
angličtina
Original language name
CTX-M-producing Escherichia coli in pigs from a Czech farm during production cycle
Original language description
We evaluated the prevalence and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in pigs during production cycle on a Czech farm with the history of previous use of ceftiofur. ESBL-producing E. Coli isolates were obtained from rectal swabs from pigs of different age groups (suckling piglets, weaned piglets, growers and sows). Collected samples were directly cultivated on MacConkey agar with cefotaxime (2 mg l(-1)), whereas intestinal swabs of slaughtered pigs and surface swabs from pig carcasses were also pre-enriched in buffered peptone water without antimicrobials before the cultivation. Clonal relationship of selected isolates was determined byXbaI pulse-field gel electrophoresis and multi-locus sequence typing. The transferability of plasmids carryingbla(CTX-M)genes was tested by conjugation experiments. From all examined samples, 141 (43 center dot 7%,n = 323) were positive for ESBL-producing E. coli. All ESBL-producing isolates showed resistance to multiple antimicrobials and were positive forbla(CTX-M)genes. Thebla(CTX-M-1)was carried by conjugative IncN/ST1 plasmids (c. 40-45 kb) while thebla(CTX-M-15)was located on conjugative F plasmids with F:18:A5:B1 formula (c. 165 kb). This study demonstrated the persistence of CTX-M-positiveE. coliisolates 2 months after banner of ceftiofur usage and indicated possible risk of transmission of these isolates to humans via the food chain.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Letters in Applied Microbiology
ISSN
0266-8254
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
369-376
UT code for WoS article
000542844500001
EID of the result in the Scopus database
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