Molecular characterization of plasmid-mediated AmpC beta-lactamase- and extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among corvids (Corvus brachyrhynchos and Corvus corax) roosting in Canada

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876604" target="_blank" >RIV/62157124:16170/18:43876604 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/18:43876604 RIV/62157124:16810/18:43876604

Result on the web

<a href="http://dx.doi.org/10.1093/femsec/fiy166" target="_blank" >http://dx.doi.org/10.1093/femsec/fiy166</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsec/fiy166" target="_blank" >10.1093/femsec/fiy166</a>

Result language

angličtina

Original language name

Molecular characterization of plasmid-mediated AmpC beta-lactamase- and extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among corvids (Corvus brachyrhynchos and Corvus corax) roosting in Canada

Original language description

This study evaluated the carriage of AmpC and extended-spectrum beta-lactamase (ESBL) genes and associated plasmids in faecal bacteria of Canadian corvids. Faecal samples from 449 birds in five roosting sites across Canada were analyzed using selective media, screening for AmpC and ESBL genes by PCR, and sequencing. Genomic relatedness was determined by PFGE and MLST. Plasmid mobility was studied by conjugation and transformation experiments, followed by plasmid typing. In total, 96 (21%, n = 449) cefotaxime-resistant Escherichia coli and three (0.7%) Klebsiella pneumoniae isolates were identified. ESBL genes bla(CTX-M-1) (n = 3), bla(CTX-M-14) (n = 2), bla(CTX-M-32) (n = 2) and bla(CTX-M-124) (n = 1) were detected in eight E. coli isolates, whereas bla(SHV-2) (2) was found in two K. pneumoniae. E. coli isolates contained bla(CMY-2) (n = 83) and bla(CMY-42) (n = 1). The high genetic diversity of the isolates and presence of clinically important E. coli ST69 (n = 1), ST117 (n = 7) and ST131 (n = 1) was revealed. AmpC genes were predominantly carried by plasmids of incompatibility groups I1 (45 plasmids), A/C (10) and K (7). The plasmid IncI1/ST12 was most common and found in diverse E. coli STs in all sites. Highly diverse E. coli isolates containing AmpC and ESBL genes, including clinically important clones and emerging plasmids, are in circulation throughout Canadian wildlife.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Microbiology Ecology

ISSN

0168-6496

e-ISSN

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Volume of the periodical

94

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000453660500009

EID of the result in the Scopus database

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