Plasmid-mediated resistance to cephalosporins and fluoroquinolones in various Escherichia coli sequence types isolated from rooks wintering in Europe

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873859" target="_blank" >RIV/62157124:16270/15:43873859 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/15:43873859 RIV/62157124:16810/15:43873859

Result on the web

<a href="http://dx.doi.org/10.1128/AEM.02459-14" target="_blank" >http://dx.doi.org/10.1128/AEM.02459-14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.02459-14" target="_blank" >10.1128/AEM.02459-14</a>

Result language

angličtina

Original language name

Plasmid-mediated resistance to cephalosporins and fluoroquinolones in various Escherichia coli sequence types isolated from rooks wintering in Europe

Original language description

Extended-spectrum-beta-lactamase (ESBL)-producing, AmpC beta-lactamase-producing, and plasmid-mediated quinolone resistance (PMQR) gene-positive strains of Escherichia coli were investigated in wintering rooks (Corvus frugilegus) from eight European countries. Fecal samples (n = 1,073) from rooks wintering in the Czech Republic, France, Germany, Italy, Poland, Serbia, Spain, and Switzerland were examined. Resistant isolates obtained from selective cultivation were screened for ESBL, AmpC, and PMQR genes by PCR and sequencing. Pulsed-field gel electrophoresis and multilocus sequence typing were performed to reveal their clonal relatedness. In total, from the 1,073 samples, 152 (14%) cefotaxime-resistant E. coli isolates and 355 (33%) E. coli isolates with reduced susceptibility to ciprofloxacin were found. Eighty-two (54%) of these cefotaxime-resistant E. coli isolates carried the following ESBL genes: bla(CTX-M-1) (n = 39 isolates), bla(CTX-M-15) (n = 25), bla(CTX-M-24) (n = 4), bla(TEM-52) (n = 4), bla(CTX-M-14) (n = 2), bla(CTX-M-55) (n = 2), bla(SHV-12) (n = 2), bla(CTX-M-8) (n = 1), bla(CTX-M-25) (n = 1), bla(CTX-M-28) (n = 1), and an unspecified gene (n = 1). Forty-seven (31%) cefotaxime-resistant E. coli isolates carried the bla(CMY-2) AmpC beta-lactamase gene. Sixty-two (17%) of the E. coli isolates with reduced susceptibility to ciprofloxacin were positive for the PMQR genes qnrS1 (n = 54), qnrB19 (n = 4), qnrS1 and qnrB19 (n = 2), qnrS2 (n = 1), and aac(6')-Ib-cr (n = 1). Eleven isolates from the Czech Republic (n = 8) and Serbia (n = 3) were identified to be CTX-M-15-producing E. coli clone B2-O25b-ST131 isolates. The widespread occurrence of highly diverse ESBL-and AmpC-producing and PMQR gene-positive E. coli isolates, including the clinically important multiresistant ST69, ST95, ST117, ST131, and ST405 clones, was demonstrated in rooks wintering in various European countries.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

—

Volume of the periodical

81

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

648-657

UT code for WoS article

000347377500021

EID of the result in the Scopus database

—