Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F17%3A43875830" target="_blank" >RIV/62157124:16170/17:43875830 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/17:43875830 RIV/62157124:16810/17:43875830
Result on the web
<a href="http://dx.doi.org/10.1111/1462-2920.13722" target="_blank" >http://dx.doi.org/10.1111/1462-2920.13722</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1462-2920.13722" target="_blank" >10.1111/1462-2920.13722</a>
Alternative languages
Result language
angličtina
Original language name
Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA
Original language description
American crow (Corvus brachyrhynchos) faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 faecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays. In total, 78 (13%, n=590) cefotaxime-resistant isolates were obtained, of which 66 and 12 displayed AmpC and ESBL phenotypes, respectively. Fifty-four AmpC-producing isolates carried bla(CMY-2). Isolates producing ESBLs contained genes bla(CTX-M-27) (5 isolates), bla(CTX-M-15) (4), bla(CTX-M-14) (2) and bla(CTX-M-1) (1). Ninety isolates (15%, n=590) with reduced susceptibility to ciprofloxacin were obtained, among which 14 harboured PMQR genes aac(6)-Ib-cr (4 isolates), qnrB19 (3), qnrS1 (2), qnrA1 (2), qnrB2 (1), qnrB6 (1) and qnrD3 (1). High genetic diversity was revealed by PFGE and MLST. Epidemiologically important E. coli clones (e.g., ST131, ST405) were identified. Plasmids carrying bla(CMY-2) were assigned predominantly to IncA/C (8 plasmids), IncI1/ST23 (5) and IncI1/ST12 (3). The study demonstrates a widespread occurrence of E. coli with ESBL, AmpC and PMQR genes associated with clinically important multidrug-resistant clones and epidemic plasmids, in American crows.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Microbiology
ISSN
1462-2912
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
2025-2036
UT code for WoS article
000400029800022
EID of the result in the Scopus database
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