A complex approach to a complex problem: the use of whole-genome sequencing in monitoring avian-pathogenic Escherichia coli - a review

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F20%3A43878999" target="_blank" >RIV/62157124:16170/20:43878999 - isvavai.cz</a>

Result on the web

<a href="https://actavet.vfu.cz/media/pdf/actavet_2020089030273.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2020089030273.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089030273" target="_blank" >10.2754/avb202089030273</a>

Result language

angličtina

Original language name

A complex approach to a complex problem: the use of whole-genome sequencing in monitoring avian-pathogenic Escherichia coli - a review

Original language description

Infections associated with Escherichia coli are responsible for immense losses in poultry production; moreover, poultry products may serve as a source of pathogenic and/or resistant strains for humans. As early as during the first hours of life, commercially hatched chickens are colonized with potentially pathogenic E. coli from the environment of hatcheries. The source of contamination has not been quite elucidated and the possibility of vertical spread of several avian pathogenic E. coli (APEC) lineages has been suggested, making the hatcheries an important node where cross-contamination of chicken of different origin can take place. The recent technological progress makes the method of whole-genome sequencing (WGS) widely accessible, allowing high-throughput analysis of a large amount of isolates. Whole-genome sequencing offers an opportunity to trace APEC and extended-spectrum/plasmid-encoded AmpC beta-lactamases-producing E. coli (ESBL/pAmpC-E.coli) along the poultry processing chain and to recognize the potential pathways of &quot;epidemic&quot; sequence types. Data from WGS may be used in monitoring antimicrobial resistance, comparative pathogenomic studies describing new virulence traits and their role in pathogenesis and, above all, epidemiologic monitoring of clonal outbreaks and description of different transmission routes and their significance. This review attempts to outline the complexity of poultry-associated E. coli issues and the possibility to employ WGS in elucidating them.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QK1810462" target="_blank" >QK1810462: Manipulation of the intestinal microbiota of pigs and poultry to reduce E. coli infections and use of antimicrobials</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Volume of the periodical

89

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

273-282

UT code for WoS article

000607909000010

EID of the result in the Scopus database

—