Genomic analysis of Escherichia coli isolates from a chicken hatchery
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880357" target="_blank" >RIV/62157124:16170/22:43880357 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/22:43880357
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Genomic analysis of Escherichia coli isolates from a chicken hatchery
Original language description
E. coli is one of the most important bacterial pathogens in commercial poultry. Recent research shows that pathogenic poultry strains (APEC) form a dynamic variable population in which "risk" genotypes and clonal lines play an important role. These can also spread vertically from parent farms to offspring, potentially from poultry products to consumers. Hatcheries appear to be a key site of cross-contamination and primary colonization of newly hatched chickens. Samples of shells and dust were taken from hatchery boxes in a commercial hatchery in the years 2020-21. Based onpreliminary characterization, 74 E. coli isolates were selected for whole genome sequencing by Illumina. The acquired data were analyzed by available tools to obtain a complex genetic characterization (VirulenceFinder, ResFinder, SeroTypeFinder, PlasmidFinder) and phylogeneticanalysis (MLST, pMLST). In contrast to previously analyzed clinical isolates, the B1 phylogenetic group dominated among the hatchery population, with a high prevalence of ST155 and ST162. Typical APEC genotypes, especially ST23 and ST117, were detected sporadically, which provestheir occurrence in the hatchery environment, where they readily colonize day-old chicks. Repeated detection could indicate their persistence in parent flocks or their spread between farms, presumably through hatcheries. Whole genome sequencing makes a fundamental tool for monitoring the occurrence of risk lines in the production chain of commercial poultry. Only long-term sampling at different levels of the pyramid with detailed genetic analysis will provide sufficient data to assess the significance of E. coli risk clonal lines in poultry.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů