Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F23%3A43880609" target="_blank" >RIV/62157124:16170/23:43880609 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0165242723000995" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0165242723000995</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetimm.2023.110645" target="_blank" >10.1016/j.vetimm.2023.110645</a>

Result language

angličtina

Original language name

Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses

Original language description

This study is the first to provide information on the lymphocyte subpopulations in peritoneal effusions in horses. Peritoneal transudates (n = 12), peritoneal exudates (n = 6) and a pleural exudate (n = 1) were analyzed. The total nucleated cell count (TNCC), total protein (TP) and matrix metalloproteinase-9 (MMP-9) concentration determined by ELISA were measured and routine cytological evaluation was performed. CD3, CD4, CD8 and CD21 positive cells were detected by flow cytometry. A higher percentage of neutrophils (P &lt; 0.05) and higher MMP-9 (P &lt; 0.01) levels were found in exudates. A higher percentage of macrophages (P &lt; 0.05) and lymphocytes (P &lt; 0.01) were found in transudates. CD4 + lymphocytes were the most common lymphocyte subpopulation in all samples. CD21 + lymphocytes were the least common in all samples. A large variability in the percentage of CD21 + lymphocytes was found in exudates. The percentage of CD21 + lymphocytes positively correlated with the level of total protein (r = 0.5704, P &lt; 0.05). The correlation was even stronger in the group of exudates. The percentages of lymphocyte subpopulations did not correlate with the level of MMP-9 or with cytological findings. The level of MMP-9 positively correlated with the percentage of neutrophils (r = 0.4980, P &lt; 0.05), the level of TP (r = 0.7855, P &lt; 0.01) and TNCC (r = 0.6129, P &lt; 0.01). A significantly higher level of MMP-9 was detected in euthanized horses than in horses that survived (P &lt; 0.05). However, it was shown that the level of MMP-9 in the peritoneal fluid can change significantly in a short time. More studies on repeated abdominocentesis could contribute to elucidating the role of MMP-9 as a prognostic indicator.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Immunology and Immunopathology

ISSN

0165-2427

e-ISSN

1873-2534

Volume of the periodical

263

Issue of the periodical within the volume

September

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

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UT code for WoS article

001064747800001

EID of the result in the Scopus database

2-s2.0-85169846692