Comparative genomic analysis of ESBL/AmpC Betalactamase-Producing E. coli from Czech Diary Calves
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F24%3A43881640" target="_blank" >RIV/62157124:16170/24:43881640 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/24:43881640
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparative genomic analysis of ESBL/AmpC Betalactamase-Producing E. coli from Czech Diary Calves
Original language description
This study analyzed 68 cefotaxime-resistant Escherichia coli isolates from calves on 16 Czech dairy farms and 15 isolates from farm workers to assess sequence types (STs), resistance and virulence genes, and zoonotic risks. Whole-genome sequencing (WGS) and bioinformatics tools were used for genetic characterization and phylogenetic analysis. Most calf isolates were multidrug-resistant, predominantly encoding blaCTX-M-14 (44.1%), blaCTX-M-1 (33.8%), blaCTX-M-8 (4.4%), and blaCTX-M-15 (16.2%). They harbored diverse plasmids (IncF, IncI1, IncB/O/K/Z, IncY) and primarily belonged to phylogenetic group A (51.5%), followed by B1 (23.5%), with lower representation of groups C, D, G, and E. One isolate carrying the IncI2-associated mcr-1 gene was detected. ST10 was the dominant sequence type (26.5%), with a zoonotic cluster (ST10-O101:H9) found in both calves and a farm worker. Another shared cluster involved ST34-O68:H30. Several isolates were potential zoonotic pathogens, including ExPEC (ST117, ST88, ST69) and STEC/EPEC strains carrying stx1, stx2, and/or the locus of enterocyte effacement (LEE). While genetic diversity was observed among calves from the same farm, some farms were dominated by clonal clusters. The observed strain diversity and gene combinations highlight the complexity of E. coli evolution and transmission dynamics. The presence of shared strains between calves and farm personnel further supports the risk of zoonotic transmission.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů