Genomic analysis of extended-spectrum beta-lactamase-producing E. coli from Czech diary calves and their caretakers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F25%3A43882544" target="_blank" >RIV/62157124:16170/25:43882544 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/25:10496545 RIV/62157124:16810/25:43882544

Result on the web

<a href="https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1552297/full" target="_blank" >https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1552297/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2025.1552297" target="_blank" >10.3389/fvets.2025.1552297</a>

Result language

angličtina

Original language name

Genomic analysis of extended-spectrum beta-lactamase-producing E. coli from Czech diary calves and their caretakers

Original language description

Introduction The increasing prevalence of antimicrobial resistance in livestock, particularly the dissemination of extended-spectrum beta-lactamase-producing Escherichia coli, poses a significant zoonotic and public health risk. This study investigates the genomic characteristics of cefotaxime-resistant E. coli isolates from dairy calves across 23 Czech farms and their caretakers.Materials and methods Bacteriological cultivation on McConkey agar with cefotaxime was used for their isolation, susceptibility to selected antibiotics was determined by disc diffusion method, production of extended-spectrum beta-lactamases (ESBL) was demonstrated by double disc synergy test. The PCR was applied to confirm the presence of selected genes encoding resistance to some beta-lactams and genes encoding resistance to quinolones carried on plasmids. Using whole-genome sequencing, we evaluated resistance genotypes, sequence types, serotypes, plasmid replicons, and virulence genes.Results and discussion Among 266 rectal samples obtained from the calves, 128 (48%) harbored cefotaxime-resistant E. coli. Whole-genome analysis revealed blaCTX-M genes in 91% (116/128) of isolates, with blaCTX-M-14 (44%) and blaCTX-M-1 (34%) being the dominant variants. Other beta-lactamase gene blaTEM-1b was found in 40% (51/128) of isolates. Notably, no cephamycin resistance genes have been identified. The plasmid-mediated quinolone resistance (PMQR) gene qnrS1 was present at 21% (27/128) of isolates. The colistin resistance gene mcr-1 was found in a single ST2325 isolate. Sequence typing revealed significant clonal diversity, with 21 different STs detected among 68 sequenced isolates. ST10 was the most prevalent (27%), followed by ST69 (12%), ST29 (7%) and others. The phylogenetic distribution showed a predominance of commensal groups A (54%) and B1 (21%). The most common serotypes included O101:H9 (21%), O15:H18 (12%), H12, and O70:H11 (7%). Analysis of plasmid content revealed a complex distribution of 18 distinct plasmid replicon types, especially IncF, followed by Col-type and IncI1-type plasmids. Cross-species transmission was indicated by the detection of clonal strains shared between calves and caretakers, notably ST10-O101:H9 and ST34-O68:H30. The prevalence of high-risk clones and the presence of mobile resistance elements underscore the urgent need for stringent monitoring, antimicrobial stewardship, and improved biosecurity measures in livestock environments like increased caution and personal hygiene of animal handlers to mitigate the spread of resistant E. coli between animals and humans.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Veterinary Science

ISSN

2297-1769

e-ISSN

2297-1769

Volume of the periodical

12

Issue of the periodical within the volume

12.5.2025

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

nestránkováno

UT code for WoS article

001451875000001

EID of the result in the Scopus database

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