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Extended spectrum beta-lactamase and fluoroquinolone resistance genes and plasmids among Escherichia coli isolates from zoo animals, Czech Republic.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F13%3A43872414" target="_blank" >RIV/62157124:16270/13:43872414 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16170/13:43872414 RIV/62157124:16810/13:43872414

  • Result on the web

    <a href="http://dx.doi.org/10.1111/1574-6941.12149" target="_blank" >http://dx.doi.org/10.1111/1574-6941.12149</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/1574-6941.12149" target="_blank" >10.1111/1574-6941.12149</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Extended spectrum beta-lactamase and fluoroquinolone resistance genes and plasmids among Escherichia coli isolates from zoo animals, Czech Republic.

  • Original language description

    Commensal Escherichia coli isolates from healthy zoo animals kept in Ostrava Zoological Garden, Czech Republic, were investigated to evaluate the dissemination of extended-spectrum beta-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes. A total of 160 faecal samples of various animal species were inoculated onto MacConkey agar with cefotaxime (2 mg L-1) or ciprofloxacin (0.05 mg L-1) to obtain ESBL-or PMQR-positive E. coli isolates. Clonality of E. coli isolates was investigated bymultilocus sequence typing and pulsed-field gel electrophoresis. Plasmids carrying ESBL or PMQR genes were typed by PCR-based replicon typing, plasmid multilocus sequence typing and restriction fragment length polymorphism. Forty-nine (71%, n = 69) cefotaxime-resistant and 15 (16%, n = 94) ciprofloxacin-resistant E. coli isolates harboured ESBL or PMQR genes. Isolates were assigned to 18 sequence types (ST) and 20 clusters according to their macrorestriction patterns by pulsed-field gel

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FEMS Microbiology Ecology

  • ISSN

    0168-6496

  • e-ISSN

  • Volume of the periodical

    85

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    604-611

  • UT code for WoS article

    000323200400016

  • EID of the result in the Scopus database