Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica).

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F09%3A00002134" target="_blank" >RIV/62157124:16270/09:00002134 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica).

Original language description

Metallothioneins (MT) play a key role in maintaining the homeostasis of essential metals and in protecting of cells against metal toxicity as well as cell oxidative damaging. The aim of this work is to propose a new approach for processing a biological sample for analysis of thiols including metallothioneins. Moreover, the proposed procedure is tested on quantification of MT and total thiol content in blood serum of pig (Sus scrofa domestica), which has not previously been performed. The blood serum (10ml) was collected and transferred to 0.2 M phosphate buffer (990 ml). The sample (100 x diluted) was placed in a thermomixer, where heat denaturation of most of the proteins proceeded. The processed blood serum sample was electrochemically measured to determine total content of thiols (cysteine, glutathione, metallothionein and other low molecular thermostable thiols) and content of MT. The average level of the thiols and MT were estimated as 165 +-20 mM and 5.2 +- 0.6 mM, respectively.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Natura Croatica

ISSN

1330-0520

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

4

Country of publishing house

HR - CROATIA

Number of pages

9

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—