Electrochemical determination of metallothionein in the domestic fowl

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F09%3A00002133" target="_blank" >RIV/62157124:16270/09:00002133 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical determination of metallothionein in the domestic fowl
Original language description
Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM(standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 ?M. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content o
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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