Identification of lactobacilli isolated from food by PCR and MALDI-TOF MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002723" target="_blank" >RIV/62157124:16270/10:00002723 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
čeština
Original language name
Identifikace laktobacilů izolovaných z potravin pomocí PCR a MALDI-TOF MS
Original language description
The aim of this study was to compare two methods for the species identification. 175 strains of lactobacilli isolated from milk and meat products (n = 139) were identified by genotypic and chemotaxonomic methods: the polymerase chain reaction (PCR) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using the PCR method strains were differentiated into 10 species and into 16 species by MALDI-TOF MS. The total agreement of both methods has been observed infive species (L. curvatus, L. delbrueckii, L. johnsonii, L. plantarum a L. sakei).
Czech name
Identifikace laktobacilů izolovaných z potravin pomocí PCR a MALDI-TOF MS
Czech description
The aim of this study was to compare two methods for the species identification. 175 strains of lactobacilli isolated from milk and meat products (n = 139) were identified by genotypic and chemotaxonomic methods: the polymerase chain reaction (PCR) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using the PCR method strains were differentiated into 10 species and into 16 species by MALDI-TOF MS. The total agreement of both methods has been observed infive species (L. curvatus, L. delbrueckii, L. johnsonii, L. plantarum a L. sakei).
Classification
Type
D - Article in proceedings
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník z XII. konference mladých vědeckých pracovníků
ISBN
978-80-7305-104-4
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
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Publisher name
Ústav hygieny a technologie mléka, FVHE; Ústav mikrobiologie a imunologie, FVL, VFU Brno
Place of publication
Brno
Event location
Veterinární a farmaceutická univerzita Brno
Event date
Jan 1, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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