Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F12%3A43871335" target="_blank" >RIV/62157124:16270/12:43871335 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/12:00062215
Result on the web
<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2012.07.029" target="_blank" >http://dx.doi.org/10.1016/j.ijfoodmicro.2012.07.029</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2012.07.029" target="_blank" >10.1016/j.ijfoodmicro.2012.07.029</a>

Result language
angličtina
Original language name
Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS
Original language description
Lactobacilli are bacteria with important implications in food and feed fermentation. Detailed knowledge of the lactobacilli composition is of high relevance to food and health control, various industrial or biotechnological applications, etc., but accurate identification of the Lactobacillus species is not an easy task. In this study, three methods, i.e. polymerase chain reaction (PCR), amplified 16S rDNA restriction analysis with restrictase MseI (16S-ARDRA), and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were evaluated for their capabilities to identify Lactobacillus species. After comparison of the three methods on a set of 19 type/reference Lactobacillus strains, 148 strains isolated from dairy and meat products were characterized by PCR and MALDI-TOF MS. The strains were differentiated into nine and ten lactobacilli species by PCR and MALDI-TOF MS, respectively, with nine of these species being congruent. The success rates of sp
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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