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Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F12%3A43871277" target="_blank" >RIV/62157124:16270/12:43871277 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

  • Original language description

    The occurrence of tetracycline resistance determinants in Escherichia coli recovered from raw cow's milk from the same farm sampled in two different time periods five years apart was investigated in this study. Detection of E. coli was performed and evaluated according to ISO 16649-2 and antibiotic resistance was screened by disk diffusion method. The polymerase chain reaction (PCR) was used to detect selected genes encoding resistance to tetracycline - tetA, tetB, tetC, tetD, and tetG. Of 89 samples ofraw cow's milk, 84 (94.4%) were positive for E. coli. In total, 102 isolates were obtained. Fifty (49.0%) of these isolates were resistant to tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010-2011was tetA (81.3 %),while tetB was most often (86.5%) found in isolates from 2005-2006. Neither the tetC, tetD, and tetG genes nor the co-occurrence of tetA and tetB genes were detected. None of the monitored genes was detected in two tetracycline-resistant

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GM - Food industry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Microbiology, Biotechnology and Food Sciences

  • ISSN

    1338-5178

  • e-ISSN

  • Volume of the periodical

    1

  • Issue of the periodical within the volume

    February

  • Country of publishing house

    SK - SLOVAKIA

  • Number of pages

    8

  • Pages from-to

    777-784

  • UT code for WoS article

  • EID of the result in the Scopus database